Molecular Biology Core
Molecular Biology Core (MBC) through the support of NIH-NCRR (1999 -2013) and NIH/ NIMHD (2014-2017) funded RCMI program is instrumental in the development and establishment of cellular and molecular biology research infrastructure at Texas Southern University (TSU). It is the integral part of TSU RCMI funded biomedical research program that has been playing significant role since its inception in 1999 in:
- Advancing biomedical research of TSU investigators to address health disparities
- Hiring of new faculty to increase research capacity and capabilities
- Introducing graduate program in Pharmaceutical Sciences.
- Expanding research infrastructure into different cores to satisfy contemporary research disciplines.
- Promoting faculty development through mentorship and training.
- Enhancing collaborative research.
- Stimulating grant applications.
Services provided by the Molecular Biology core from 2000 to 2013 resulted in: over 50 publications, more than 70 presentations and about 40 grant applications of which 6 received funding support.
MBC continues to provide cellular and molecular biology resources and services to faculty and research staff to:
- Enhance the caliber of their research with use of contemporary technologies;
- Obtain preliminary data to publish manuscripts, deliver presentation and submit grant applications.
- Establish collaborations within and outside TSU.
Available Resources and Services
The following resources and services through hands-on training are available to investigators from the MBC.
Cell culture: Include CO2 incubators, biosafety cabinets, water bath and microscope, and hands-on training to maintain cells and tissues in culture.
Imaging: Include, Bio-Rad Multipurpose Phospho Imager Pro Plus System for imaging of DNA, RNA, and protein gels, blots, or x-ray films for genotyping and monitoring cDNA synthesis, cloning/subcloning and gene knock-down and gene knock-in analysis. Assessing changes in protein levels or studying signal transduction pathways in cells or tissues/organs from animals or other organisms. LI-COR Odyssey CLx system for filmless western blotting, cell-based assays, protein arrays, gel shift assays and tissue section imaging with near-infrared fluorescence.
Real Time PCR: Include Vii 7 Real-Time PCR system from Life Technologies for studying gene expression changes, amplification of gene sequence of interest, microRNA analysis, PCR-based gene array screening and ChIP assays
Microscopy: Include, Nikon TS500 fluorescent microscope to determine protein expression, cellular localization, co-localization and intracellular protein shuttling in response to different stimuli and monitoring post-translation modifications of proteins, Detection of changes in expression of proteins or genes in target cells/organs/tissues by immunocytochemistry, immunohistochemistry and in-situ hybridization.
Proteomics: Include Bio-Rad and Hoefer protein electrophoresis systems to separate and transfer proteins to membranes for identifying, purifying, quantitating target proteins and monitoring post-translation modification by phosphorylation, acetylation and methylation. Protein immunoprecipitation to study protein-protein interactions. EMSA to study protein-DNA interaction. Plate reader for protein quantitation, enzyme assays and ELISA assays.