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Dr. Jade Clement
Research Objective: Identification of Major Space Gene
Identify major cellular genes whose activity may be altered by microgravity. Since different
organ or tissue systems may be differentially affected by microgravity, various cell lines of different linage or tissue origin will be used to generate a comprehensive information database of cellular activity alteration associated with microgravity. The results of this research may provide a scientific basis for developing therapeutic intervention aimed at more effectively managing the adverse health effects of space flight.
Training/Instructional Capability:
- Course areas: Molecular Biology, Bio-Organic Chemistry, General Biology, Developmental Biology, Tumor Biology, Biological Chemistry, Microbiology, Immunology, Anatomy & Physiology, Molecular and Cellular Toxicology
- Lab areas: molecular engineering (recombinant DNA), transformation, plasmid and genomic DNA isolations, RE digestion, subclonning, PCR, Southern blotting, sequencing, transfection (stable and transient), cDNA array, RNA isolations (total, N/C, PA+ ), Northern blotting, RT-PCR, intron lariat identification and debranching, primer extension, protein isolation, Western blotting, ELISA, apoptosis, MTT, cell culture
Selected Publications:
- Clement JQ, Maiti, S, Wilkinson MF (2001) Localization and stability of introns spliced from the Pem homeobox gene. Journal of Biological Chemistry. 276 (20): 16919-30.
- Clement JQ, Wilkinson MF (2000) Rapid induction of nuclear transcripts and inhibition of intron decay in response to polymerase II inhibitor DRB. Journal of Molecular Biology. 299 (5):1179-91.
- Clement JQ, Qian L, Kaplinsky N, Wilkison MF (1999) The stability and fate of a spliced intron from vertebrate cells. RNA. 5 (2):206-20.
- Misteli T, Caceres JF, Clement JQ, Krainer AR, Wilkinson MF, Spector DL (1998) Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. Journal of Cell Biology. 143 (2):297-307.
Recent Professional Conferences Attended:
- The Welch Foundation 45th Conference on Chemical Research, October 29-30, 2001
- Experimental Biology 2002, FASEB, New Orleans, Louisiana, April, 2002
- RCMI Spring Symposium, Jackson, Mississippi, April 2002
- 2003 NASA Cell Science Conference, Houston, Texas, February 20-23, 2003
Recent Training Workshops Attended: (TSU or External)
- Basic Radiation Training Course (TSU), 11/09/01
- Research Involving Human Subjects (TSU), 11/14/01
- Proposal Writing Workshop (TSU), 2/13/02~2/15/02
- Developing Multimedia Presentations (HCC), April 2002
- OMB Circular A-133 Training, 10/25/02
- Protection of Human Subjects in Research and Other Compliance Issues (TSU), 11/1/02
- Win-Win Negotiation ~ Resolving & Preventing Conflicts (TSU), 11/7/02
- Dealing with Difficult People in the Workplace (TSU), 11/20/02
- OMB Circular A-21 & Pre-award Process Training (TSU), 12/11/02
- Charter Day Workshop: No Child Left Behind (TSU) 03/06/03
- HIPAA Workshop (TSU) 03/11/03
- HR Training Workshop on Effective Management (TSU) 03/20/03
List of Professional Activities:
- Regular Member of American Society for Biochemistry and Molecular Biology
- Regular Member of the American Society for Microbiology
List of Community and University Services:
- Chair, Recombinant DNA Committee, 2001~present
- Animal Safety Committee, 2001~present
- Institutional Compliance Committee, 2001~present
- Research Coordinator, College of Science and Technology, 2001~2002
NASA Research Center For Biotechnology and Environmental Health
http://www.tsu.edu/academics/science/research/nasa.asp
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